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Abstract

October‐December 2017 | Vol. 10 | Issue 4 | Page No 15-16
How Far Have We Progressed in Cutting and Pasting the Genome
Dhanya Lakshmi N
Department of Medical Genetics, Nizam’s Institute of Medical Sciences, Hyderabad
Address for Correspondence Email: dhanyalakshmi@gmail.com
 
Abstract
CRISPR/cas9 was proposed to have the ability to correct disease- causing mutations by gene editing. Previous studies done showed that the efficacy of homology directed repair (HDR) was only 10% when CRISPR/ cas9 was used in triploid human embryos. In this study, the authors have performed cas9-mediated gene editing in diploid human embryos with point mutation in the G6PD and HBB genes. They have shown that there was HDR in 100% of embryos (two embryos were used) with a mutation in G6PD. Out of this, one embryo showed complete correction but the other became a mosaic with 50% cells demonstrating 4bp deletion near the mutation. In this study 50% HDR for HBB gene was demonstrated. Only one corrected embryo was free of off-targeting. The authors concluded that the use of CRISPR/Cas9-mediated gene editing in reproductive clinics is not a current option due to both ethical and technical issues like safety and mosaicism. The authors suggested that CRISPR/Cas9 system could be used in human zygotes to study gene function in preimplantation development.
 
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