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Clinical Vignette
Metabolites | Observed values | Reference Range
|
Urinary total porphyrin/ Creatinine | 3428.13 ug/g creat | <175 ug/g creat |
Uroporphyrin total/ Creatinine | 2742.58 ug/g creat | <33 ug/g creat |
Uroporphyrin I isomers | 92% | 53-79% |
Uroporphyrins III isomers | 7.99% | 21-47% |
Coproporphyrin total/ Creatinine | 485.68 ug/g creat | <120 ug/g creat |
Coproporphyrin I isomers | 96.53% | 17-31% |
Coproporphyrin III | 3.47% | 69-83% |
Uroporphyrin: coproporphyrin ratio | 5.65 | 0.07-0.65 |
The child was advised sun protection, protective clothing and sunscreen. Hematopoietic stem cell transplantation is the only curative therapy for CEP at present and the family was counselled about the disorder and further management.
CEP also known as ‘Günther disease’ deriving its name from the physician who first described it, is a rare disorder with approximately 200 patients reported till date (Erwin et al., 2019). It is a severe bullous porphyria characterized by deficiency of the enzyme uroporphyrinogen synthase III (UROS III) which catalyzes the conversion of hydroxymethylbilane into uroporphyrinogen III. In the deficiency of UROSIII, hydroxymethylbilane enters the non-enzymatic pathway and gets converted into uroporphyrinogen I and coproporphyrinogen I and subsequently into uroporphyrin I and coproporphyrin I. These uroporphyrins get deposited in various tissues, bone marrow, plasma, skin and urine. These compounds are photocatalytic and on exposure to long-wavelength ultraviolet (UV) light result in blister and vesicle formation and increased skin fragility as seen in our patient. In erythrocytes and bone marrow these result in hemolysis and subsequent ineffective erythropoiesis and erythroid hyperplasia (Erwin et al., 2019).
The clinical phenotype of CEP is quite variable ranging from in-utero presentation to mild adult phenotype. The most common clinical presentation is photosensitivity with the formation of bullous lesions which heals with scarring and hyperpigmentation. In severe cases, the loss of digits of hands and feet, also known as photo-mutilation, has been reported. Other features include corneal scars, ulcers, red or dark colored urine, erythrodontia and hemolytic anemia. Earliest sign in infancy is red staining of diapers with urine due to accumulated porphyrins (Erwin et al., 2019).
Other differentials which need to be considered include other bullous porphyrias and photocutaneous disorders like pseudoporphyria, and epidermolysis bullosa. The other bullous porphyrias which need to be considered include HEP, PCT, VP and HCP (Figure 2). HEP, caused by biallelic variants in UROD has a childhood-onset with similar phenotype whereas PCT, caused by heterozygous variant in UROD, has an adult-onset and a milder presentation. Both are differentiated through urine and plasma porphyrin analysis. Pseudoporphyria is a drug-induced bullous disorder seen in children with juvenile rheumatoid arthritis who are on nonsteroidal anti-inflammatory drugs (NSAIDs). In pseudoporphyria, levels of porphyrins in the urine, plasma, and stool are in the normal range. Patients with epidermolysis bullosa have bullous lesions, which usually present in the neonatal period or early infancy and the bullae usually heal without scar formation.
For bullous cutaneous porphyrias, the first-line investigations include measurement of total porphyrins in urine, plasma or stool, followed by high-performance liquid chromatography (HPLC) for typing the porphyria based on the quantity of porphyrins elevated in urine (Figure 2). CEP is characterized by the accumulation of uroporphyrin I and coproporphyrin I as seen in our patient. Initial screening can also be done with spectrofluorometer which gives specific plasma fluorescence emission peaks for VP and CEP at 626 nm and 615-618 nm respectively (Rigor et al., 2019). Further confirmation can be done with molecular testing.
CEP is caused by either biallelic pathogenic variants in the UROS gene in 98% of the cases or on rare occasions, by hemizygous pathogenic variants in the GATA1 gene (1%). Variants including missense, nonsense, frameshift and splice site variants as well as intragenic deletions/duplications have been reported in the UROS gene. The most common UROS pathogenic variant reported in literature is p.Cys73Arg, observed in 30-40% of the cases. The clinical phenotype is largely determined by the amount of residual enzyme activity (Erwin et al., 2016; 2019).
The treatment is predominantly supportive and consists of avoidance of exposure to sunlight and artificial sources of UV light, and red blood cell transfusions for hemolytic anemia. The only curative therapy is hematopoietic stem cell transplantation, but this is also associated with significant morbidity and hence is performed only in transfusion-dependent patients or those with very severe skin manifestations. Other therapies are experimental including gene therapy, proteasome inhibitor (Bortezomib) and chaperone therapy (Ciclopirox) (Urquiza et al., 2018).
A meticulous clinical and laboratory approach is the key to early diagnosis of congenital erythropoietic porphyria, to halt further disease progression and open ways for future treatment options. Molecular testing is confirmatory and is necessary for family counseling and management and prevention of recurrence in future pregnancies.
1. Erwin A, et al. Porphyrias Consortium of the NIH-Sponsored Rare Diseases Clinical Research Network. Congenital Erythropoietic Porphyria.2013 Sep 12 [updated 2016 Apr 7]. In: Adam MP, Ardinger HH, Pagon RA, Wallace SE,Bean LJH, Stephens K, Amemiya A, editors. GeneReviews® [Internet]. Seattle (WA): University of Washington, Seattle; 1993-2020. Available from: http://www.ncbi.nlm.nih.gov/books/NBK154652/PubMed PMID: 24027798.
2. Erwin AL, Desnick RJ. Congenital erythropoietic porphyria: Recent advances. Mol Genet Metab 2019; 128: 288–297.
3. Katugampola RP et al. Congenital erythropoietic porphyria: a single-observer clinical study of 29 cases. Br J Dermatol 2012; 167: 901–13.
4. Richards S, et al. Standards and Guidelines for the Interpretation of Sequence Variants: A Joint Consensus Recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med 2015; 17: 405–424.
5. Rigor J, et al. Porphyrias: A clinically based approach. Eur J Intern Med 2019; 67: 24–29.
6. Urquiza P, et al. Repurposing ciclopirox as a pharmacological chaperone in a model of congenital erythropoietic porphyria. Sci Transl Med 2018; 10(459). doi: 10.1126/scitranslmed.aat7467.
7. Weiss Y, et al. Congenital erythropoietic porphyria and erythropoietic protoporphyria: Identification of 7 uroporphyrinogen III synthase and 20 ferrochelatase novel mutations. Mol Genet Metab 2019; 128: 358–362.
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